Illumina coverage calculator

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Arial,Regular" Product Information: Coverage Calculator for Illumina Sequencing Systems Arial,Regular" Illumina, Inc. • 9885 Towne Centre Drive, San Diego, CA 92121 USA • 1.800.809.4566 toll-free • 1.858.202.4566 tel • [email protected]illumina.com Current as of 10 August 2011. 25000000 15000000. Required coverage: 2X. expected abundance level: 0.5 %. 5Mbp x 2 x 100 / 0.5 = 2Gbp. A sampling size of 2Gbp is required to get a 2X coverage of a 5Mbp genome present at 0.5% abundance level. How many reads? example: 2 x 200bp read length (paired reads) samplingDepth / readLength / 2 paired reads. 2,000,000,000 bp / 200 bp / 2 = 5,000,000. Sequencing Coverage Calculator; Custom Protocol Selector; More Tools. Benefits of Gene Expression Profiling with RNA-Seq. ... At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to.
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